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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
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Data corrente: |
22/08/2022 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NICIURA, S. C. M.; BENAVIDES, M. V.; OKINO, C. H.; IBELLI, A. M. G.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; CINTIA HIROMI OKINO, CPPSE; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; ALESSANDRO PELEGRINE MINHO, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Genome-wide association study for Haemonchus contortus resistance in Morada Nova sheep. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pathogens, v. 11, n. 8, aug. 2022, 939. |
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Páginas: |
12 p. |
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DOI: |
https://doi.org/10.3390/pathogens11080939 |
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Idioma: |
Inglês |
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Conteúdo: |
Among the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification. MenosAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for... Mostrar Tudo |
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Palavras-Chave: |
Gastrointestinal nematode control; GWAS; Molecular markers; Ovine; Parasite resistance. |
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Thesagro: |
Marcador Molecular; Ovino; Parasito. |
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Thesaurus Nal: |
Genotyping. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145606/1/GenomeWideAssociation.pdf
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Marc: |
LEADER 02449naa a2200325 a 4500
001 2145606
005 2022-08-22
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/pathogens11080939$2DOI
100 1 $aNICIURA, S. C. M.
245 $aGenome-wide association study for Haemonchus contortus resistance in Morada Nova sheep.$h[electronic resource]
260 $c2022
300 $a12 p.
520 $aAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification.
650 $aGenotyping
650 $aMarcador Molecular
650 $aOvino
650 $aParasito
653 $aGastrointestinal nematode control
653 $aGWAS
653 $aMolecular markers
653 $aOvine
653 $aParasite resistance
700 1 $aBENAVIDES, M. V.
700 1 $aOKINO, C. H.
700 1 $aIBELLI, A. M. G.
700 1 $aMINHO, A. P.
700 1 $aESTEVES, S. N.
700 1 $aCHAGAS, A. C. de S.
773 $tPathogens$gv. 11, n. 8, aug. 2022, 939.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
24/11/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CARVALHO, W. A.; DOMINGUES, R.; PRATA, M. C. de A.; SILVA, M. V. G. B.; OLIVEIRA, G. C. de; GUIMARÃES, S. E. F.; MACHADO, M. A. |
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Afiliação: |
WANESSA ARAUJO CARVALHO, CNPGL; ROBERT DOMINGUES, CPPSUL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; GUILHERME CORRÊA DE OLIVEIRA, FIOCRUZ; SIMONE ELIZA FACIONI GUIMARÃES, UFV; MARCO ANTONIO MACHADO, CNPGL. |
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Título: |
Microarray analysis of tick-infested skin in resistant and susceptible cattle confirms the role of inflammatory pathways in immune activation and larval rejection. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Veterinary Parasitology, Amsterdam, v. 205, n. 1/2, p. 307-317, 2014. |
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DOI: |
10.1016/j.vetpar.2014.07.018 |
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Idioma: |
Inglês |
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Conteúdo: |
Tick bites promote activation of an inflammatory process that is influenced by bovine genetic composition and its history of previous exposure. Taurine and indicine breeds are known to differ on its immune response development against Rhipicephalus microplus. Nevertheless, further investigation about the complex molecular pathways involved in the development of immune response to tick infestation in cattle presenting the same genetic background is mandatory. The aim of this work was to access the early immune response triggered by R. microplus larvae attachment in previously selected resistant and susceptible animals in a bovine F2 population derived from Gyr (Bos indicus)×Holstein (Bos taurus) crosses. Microarray data analysis of RNA samples from tick infested skin was used to evaluate the gene expression at 0, 24 and 48h after R. microplus larvae attachment. Our experimental design allowed us to deeply explore the immune response related to R. microplus infestation avoiding the innate differences between these breeds. The differentially expressed genes found reveal networks and pathways that suggest a key role of lipid metabolism in inflammation control and impairment of tick infestation in resistant animals. Acute phase response also seems to be impaired in susceptible animals. These results provide new insights about early immune response against ticks and raise the possibility of using immunomodulation processes to improve and develop novel tools for tick control. |
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Palavras-Chave: |
Imunomodulação. |
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Thesagro: |
Bovino; Imunologia. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02311naa a2200241 a 4500
001 2000821
005 2024-02-05
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vetpar.2014.07.018$2DOI
100 1 $aCARVALHO, W. A.
245 $aMicroarray analysis of tick-infested skin in resistant and susceptible cattle confirms the role of inflammatory pathways in immune activation and larval rejection.$h[electronic resource]
260 $c2014
520 $aTick bites promote activation of an inflammatory process that is influenced by bovine genetic composition and its history of previous exposure. Taurine and indicine breeds are known to differ on its immune response development against Rhipicephalus microplus. Nevertheless, further investigation about the complex molecular pathways involved in the development of immune response to tick infestation in cattle presenting the same genetic background is mandatory. The aim of this work was to access the early immune response triggered by R. microplus larvae attachment in previously selected resistant and susceptible animals in a bovine F2 population derived from Gyr (Bos indicus)×Holstein (Bos taurus) crosses. Microarray data analysis of RNA samples from tick infested skin was used to evaluate the gene expression at 0, 24 and 48h after R. microplus larvae attachment. Our experimental design allowed us to deeply explore the immune response related to R. microplus infestation avoiding the innate differences between these breeds. The differentially expressed genes found reveal networks and pathways that suggest a key role of lipid metabolism in inflammation control and impairment of tick infestation in resistant animals. Acute phase response also seems to be impaired in susceptible animals. These results provide new insights about early immune response against ticks and raise the possibility of using immunomodulation processes to improve and develop novel tools for tick control.
650 $aBovino
650 $aImunologia
653 $aImunomodulação
700 1 $aDOMINGUES, R.
700 1 $aPRATA, M. C. de A.
700 1 $aSILVA, M. V. G. B.
700 1 $aOLIVEIRA, G. C. de
700 1 $aGUIMARÃES, S. E. F.
700 1 $aMACHADO, M. A.
773 $tVeterinary Parasitology, Amsterdam$gv. 205, n. 1/2, p. 307-317, 2014.
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Embrapa Gado de Leite (CNPGL) |
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